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Image Search Results
Journal:
Article Title: Role of JAK2 signal transductional pathway in activation and survival of human peripheral eosinophils by interferon-gamma (IFN-?)
doi: 10.1046/j.1365-2249.1999.01068.x
Figure Lengend Snippet: Effect of IL-5, IFN-γ and tumour necrosis factor-alpha (TNF-α) on eosinophil survival. Purified eosinophils were cultured in the presence of rhIL-5 (10 ng/ml; •), rhIFN-γ (1000 U/ml; □), rhTNF-α (10 ng/ml; ○) or medium only (▪) for 48 h. The viability of eosinophils was determined by examining 200 cells stained with trypan blue dye exclusion.
Article Snippet: Eosinophils (5 × 10 5 ) with recombinant human IFN-γ (rhIFN-γ; Genzyme, Boston, MA), rhIL-5 (Genzyne), or
Techniques: Purification, Cell Culture, Staining
Journal:
Article Title: Role of JAK2 signal transductional pathway in activation and survival of human peripheral eosinophils by interferon-gamma (IFN-?)
doi: 10.1046/j.1365-2249.1999.01068.x
Figure Lengend Snippet: Effect of JAK2 inhibitor on IFN-γ-induced eosinophil survival. A JAK2 inhibitor, AG-490 (10−5–5 × 10−5 m) was added to the eosinophil culture medium with rhIL-5 (10 ng/ml), rhIFN-γ (1000 U/ml), or recombinant human tumour necrosis factor-alpha (rhTNF-α; 10 ng/ml). The viability of 48 h cultured eosinophils was determined by examining 200 cells stained with trypan-blue dye exclusion.
Article Snippet: Eosinophils (5 × 10 5 ) with recombinant human IFN-γ (rhIFN-γ; Genzyme, Boston, MA), rhIL-5 (Genzyne), or
Techniques: Recombinant, Cell Culture, Staining
Journal:
Article Title: Role of JAK2 signal transductional pathway in activation and survival of human peripheral eosinophils by interferon-gamma (IFN-?)
doi: 10.1046/j.1365-2249.1999.01068.x
Figure Lengend Snippet: Effect of JAK2 inhibitor on IFN-γ-induced CD69 expression on 24 h cultured eosinophils. A JAK2 inhibitor, AG-490 (10−5–5 × 10−5 m) was added to the eosinophil culture medium with rhIL-5 (10 ng/ml), rhIFN-γ (1000 U/ml), or recombinant human tumour necrosis factor-alpha (rhTNF-α; 10 ng/ml) from time zero to 24 h. Flow cytometric analysis was then performed. The data on mean fluorescence intensity (MFI) of CD69 are shown in ratio form as described in Materials and Methods. Data are mean ± s.d. for six experiments. Significantly different from the mean value of the control: *P < 0.05; **P < 0.01.
Article Snippet: Eosinophils (5 × 10 5 ) with recombinant human IFN-γ (rhIFN-γ; Genzyme, Boston, MA), rhIL-5 (Genzyne), or
Techniques: Expressing, Cell Culture, Recombinant, Fluorescence